Genetics and molecular research 13 (1): 1160-1168 (2014) ©funpec-rp wwwfunpecrpcombr detection of genetically modified maize and soybean in. A simple dna extraction method suitable for pcr detection of genetically modified maize pcr performed with selected primers yielded reproducible detection of. A method of detecting recombinant dnas from four lines of genetically modified maize journal of food hygienic society of japan, 41, 2000, pp 137-143  h olck, a, v aïtilingom, m, d idierjean, l, r udi, k: 5'-nuclease pcr for quantitative event-specific detection of the genetically modified mon810 maisgard maize. To address this issue, it is necessary to detect genetically modified (gm) maize and establish a monitoring and regulatory system in nepal currently, nepal does not have legal regulations or labeling directives for gm maize. Indian journal of biotechnology vol 5, october 2006, pp 510-513 detection of transgenes in genetically modified soybean and maize using polymerase chain reaction.
Download citation on researchgate | detection of genetically modified maize safety-approved in korea using pcr | four lines (mon810, ga21, nk603, and tc1507)of. A detection method for gm maize 1151 grains were also provided by kfda for the monitoring of gm maize, maize for feeding, imported from the united states. European commission commission recommendation 2004/787/ec of 4 october 2004 on technical guidance for sampling and detection of genetically modified organisms and material produced from genetically modified organisms as or in products in the context of regulation (ec) no 1830/2003 off j eur union 2004, l 348, 18-26. Detection and quantification of genetically modified maize via polymerase chain reaction a thesis submitted to the graduate school of natural and applied sciences.
Real-time quantitative pcr detection of genetically modified maximizer maize and roundup ready soybean in some representative foods. Detection of genetically modified maize dna fragments in the intestinal contents of pigs fed starlink cbh351 chowdhury eh(1), mikami o, nakajima y, hino a, kuribara h, suga k, hanazumi m, yomemochi c author information: (1)safety research division, national institute of animal health, tsukuba, ibaraki 305-0856, japan. Pcr detection of genetically modiﬁed maize and soy in mildly and highly processed foods özge özgen arun, funda yılmaz, karlo muratoglu istanbul university. Transgenic maize grain is usually grown with conventional varieties on farms in the usa maize samples were mixed into non-gm maize at level of 25, 5, 1, 05, and 01% mon810 or nk603 as shown in figure 3, the detection sensitivity for mon810 and nk603 by multiplex pcr was about 05%, approximately 45 copies of genomic dna.
The increase in new gmos (genetically modified organisms) has led to specific legislation world-wide to regulate the presence and/or the amount of gmos in food, food ingredients, feeds and agriculture commodities regulatory demands of labelling and traceability of gmos in the food chain need suitable sampling protocols and analytical. 3 gordana zdjelar et al: detection of genetically modified crops in animal feed in serbia 100% canola leaf dna t45 (aocs, usa), rf3 (aocs, usa) and canola seed rt73. Detection of genetically modified maize by multiplex pcr method journal of microbiology and biotechnology 2004 dec 114(6):1150-1156. Regulation (ec) no 1830/2003, of the european parliament and of the council, concerning the traceability and labelling of genetically modified organisms and the traceability of food and feed products produced from genetically modified organisms and amending directive 2001/18/ec 15798/1/ 02 rev1.
For confirmation test of the genetically modified maize and soybean food products, nested polymerase chain reaction experiments were performed using internal primers for the detection of the e35s promoter and the hsp70 exon1/intron1 region of maize mon810 and cp4 epsps gene of soybean. Of the primer sets tested, cry 03/04 showed the best performance and led to the detection of transgenic dna in final cooked products containing amounts of bt-maize dna as low as 01. Highlights we detected gmos in 25 of 100 food samples purchased from retail shops in turkey pcr is used for both novel and species-specific sequence detection.
Anal bioanal chem 375: 985-993 huang hy, pan tm (2004) detection of genetically modified maize mon810 and nk603 by multiplex and real-time polymerase chain acknowledgements reaction methods j agric. The determination of the presence of genetically modified plant material by the detection of expressed genetically engineered proteins using lateral flow protein. The gmo (genetically modified organism) labeling system on raw materials has been in korea since march 2001, and genetically modified organisms (gmos)-derived foods. Detection of genetically modified maize food products by the polymerase chain reaction ciencia y tecnología alimentaria, vol 5, núm 3, 2006, pp 175-181.